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Bio-ethanol Production from Corncob Residue Using Mixed Culture of Cellulolytic Bacteria and Yeast
Researcher : T. C. Sunarti, A. Meryandini, N. Richana
Published : 2008
Group :Biofuel
Full Report : Special request
Abstract :
The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic biomassa is a part of bioethanol production process.  In this experiments delignified corncob,  its cellulose fraction and alpha cellulose were used as substrate to produce simple sugar by using three local isolates of celluloytic bacteria ((C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol.
The results shows that all isolates of cellulolytic bacteria can grow on cellulose fraction better then delignified corncob, and alpha cellulose.  But the highest hydrolytic activity produced from cellulose fraction and isolate of C4-4, liberated 3.50 g/l of total sugar.  Ethanol can be produced by mixed culture fermentation of bacteria and yeast.  But competitive growth only produced 0.39-0.47 g/l of ethanol, and organic acids 1.20-1.28%.