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Pathogenicity Characteristic of Avian Influenza Virus Indonesian Isolate in Various Poultry From 2007 to 2008
Researcher : M. H. Wibowo, K. Putri
Published : 2008
Group :Other Areas
Full Report : Special request
Abstract :
Highly pathogenic avian influenza outbreak in Indonesia has been reported in various poultry, due to H5N1 subtype. The presence of multiple basic amino acids within the cleavage site of HA glycoprotein has been identified to be associated with the pathogenicity of AIV. Based on the feature of basic amino acid in this region, AIV could be classified as high or low pathogenic avian influenza virus. Recently a specific case has been reported that some AIV isolated in 2006 in certain area in West Java showed a mutation of arginine to serine amino acid at upstream-6 position within cleavage site region. People assume that the same kind of mutation could also happen in another AIV isolates of non West Java origin. The aim of this study was to characterize the cleavage site region of haemagglutinin gene of AIV isolated from various poultry in 2007 to 2008. Isolation, identification and propagation were carried out to collect viral stock. For virus detection, reverse transcriptase PCR (RT-PCR) method on HA and N1 gene fragment were performed. Amplification target size was 246 bp for cleavage site area and 131 bp for N1. All of RT-PCR HA gene positive products were sequenced for further nucleotide analysis and to determine the nucleotide composition at the targeted fragment. The results are all AIV isolates identified as H5N1 subtype. The sequences analysis show a series of multiple basic amino acid motif -PQRESRRKKR//GL- and –PQRE-RRKKR//GL- that classified as highly pathogenic avian influenza motif. Further analyses find astonishment facts that at upstream -6 there is a deletion of arginine and also a mutation of amino acid from arginine to serine. Phylogenetic tree study among fragment HA gene in this study, rooted from A/China/GDN01/2006 were suggested that our isolates indicated from the same lineage. Our result emphasized assumption of AIV isolates mutation found not only from certain area in West Java but also from other places and the fact that AIV is continuing to evolve.