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Production and Characterization of a Recombinant Xylanase from Local Marine Thermophilic Geobacillus Stearothermophilus
Researcher : L. Sukmarini, E. Yetti
Published : 2009
Group :Other Areas
Full Report : Special request
Abstract :
A gene xylanase xynA which was isolated from local strain thermophilic Geobacillus stearothermophilus from the surround of volcanic crater, marine Tanjung Api, Poso, Indonesia, has been cloned. The xynA gene consists of a-1221 bp open reading frame, encoding 407 amino acid residues including 30 residues of signal peptide. The overall amino acid sequence shares high identity with thermo-alkalinestable xylanase of G. stearothermophilus which is belonging to glycoside hydrolase family 10 (GH10). The fragment of xynA gene was subcloned using plasmid pET-21b and was expressed in Escherichia coli BL21 (DE3) pLys. The expressed recombinant protein is predicted to be ± 43 kDa of molecular weight. Unfortunately, recombinant xylanase can not be expressed well, due to the plasmid pET-21b is relatively not stable. As alternative, the further study
using known and proofed of more stable plasmid pET-32b is under investigation.
Recombinant xylanase from the local strain is suggested to have desirable characteristics of
thermostable and alkalinestable that is useful for pulp and paper industry.

Keywords: Geobacillus stearothermophilus, glycoside hydrolase 10, local strain,
thermophilic, thermo-alkalinestable, xylanase.