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Callus Induction Of Cottonii Seaweed (Kappaphycus alvarezii (Doty) Doty) Colleted From Natuna Islands, Riau Islands Province
Researcher : E. Sulistiani, D.T. Soelistyowati, Alimuddin, S. A. Yani
Published : 2010
Group :Agriculture
Full Report : Special request
Abstract :
The objective of this study was to obtain the optimal medium for callus induction from thallus explants of K. alvarezii. Before cultured, Cottonii seaweeds collected from the Natuna Islands (Riau Islands Province) were acclimatized in greenhouse and in semisterile culture in the laboratory. Sterilized explants were cultured on PES and Conwy media solidified with 0.8% Bacto agar. In each of these media were added two combinations of plant growth regulators i.e. BAP+IAA and BAP+NAA. The concentrations of BAP used were 0, 0.5, 1 mg/l, the concentrations of IAA were 0, 2.5, 5 mg/l, whereas the concentration of NAA were 0, 0.5, 1 mg/l. The result indicated that the optimal medium for callus induction was PES solidified medium supplemented with BAP 1 mg/l. Types of callus formed were (a) white compact callus, (b) white filamentous callus, (c) greenish / brownish callus. Regeneration of callus into clump of filament had been done by subcultured the callus into PES solidified medium supplemented with BAP 1 mg/l + IAA 2.5 mg/l.