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Phytochemical study and initiation of cell and organ cultures, in order to provide safe Jatropa curcas plants as the source of biofuel
Researcher : Elfahmi
Published : 2007
Group :Biofuel
Full Report : Special request
Abstract :
Jatropa curcas belongs to the Euphorbiaceae family and has a high economic value. This plant has been reported to contain toxic compounds such as curcin and phorbol ester and its derivatives. These compounds may be a problem if J. curcas will be explored as sources of biofuel. In the effort to obtain safe J. curcas plants, a research on phytochemical and initiation of cell and organ culture have been carried out. Jatropa curcos collected from different regions in Indonesia showed to contain relatively the same profile of chemical contents. Dominant compounds that were detected by GCMS are hydrocarbon such as 2-heptenal, decadienal, hexadecane, pentadecane, cyclooctane, etc, fatty acid such as octadecanoate acid, ethyl linoleate, ethyl stearate, hexadecanoate acid and steroid such as stigmasterol, fucosterol, sitosterol. No phorbol ester and its derivatives have been detected by the CGMS method. Callus and suspension cultures of J. curcas have been establised to be used for further investigation. The secondary metabolities of original plant (seed, leaf) have different profiles compared to the callus and cell suspension. It could be concluded that the callus and cell suspension produced the additional compounds. In addition, some compounds from original plants were not found in the callus and suspension cultures.