Screening of Essential Oil Amended Coatings to Minimize Damage on Mango for Export Commodity Due to Colletotrichum gloeosporioides and Lasiodiplodia theobromae Attacks
M. M. Novianti, D. Dewantara, R. Rosita*, H. Affandi

Source: SEAMEO BIOTROP's Research Grant | 2013

Abstract:

The aim of this study was to evaluate the fungicidal effect of the clove, lemon, betel leaves, garlic, galangal and thyme leaves essential oils studies against C. gloeosporioides  and  L. theobromae, and to determine the possibility of incorporating them in coatings to control postharvest diseases of mangoes gedong gincu and arumanis as export commodities.  For in vitro studies, essential oils were tested to evaluate their effect on mycelial growth of  C. gloeosporioides  and L. theobromae during given incubation times.  Results indicated that clove oil has the highest fungicidal effect than other essential oils.  It cause by the high content of flavonoids in eugenol compound, tannins and saponin that inhibited the growth of C. gloeosporioides and L. theobromae. Differences in the concentration of essential oils (0,025; 0,050; 0,075; 0,100 % v/v) to the inhibitory effects against C. gloeosporoides and L. theobromae also showed no significant (P > 0,05).  It was also observed that concentration was not a key factor in reducing the development of these two fungi as it occurred in in vitro studies.

For in vivo tests, mangoes were dipped in the wax coating added with clove oil with concentrations 0,025; 0,050; 0,075; 0,100 % (v/v) after inoculation. Non-inoculated fruits were similarly treated.  Results indicated that application of 0.1 % clove essential oils in the wax coating proven to effectively inhibit fruit damage due to attack C. gloeosporioides and L. theobromae up to 12 days on mango infected and 16 days on mango which are picked from the tree.  The result of organoleptics test shown statistically there is no change in color, aroma, color and taste of the fruit flesh because the wax coating added by clove oil and no difference in the level of consumer preferences of a sample due to the treatment.

Keywords :  gedong gincu, arumanis, C. gloeosporioides, L. theobromae, essential oil, coating


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