Genetic Variation and Identification on Pangolin (Manis Javanica) Confiscated Using Control Region Mitochondrial DNA
Wirdateti, G. Semiadi

Source: SEAMEO BIOTROP's Research Grant | 2014


Sunda pangolin (Manis javanica) in Indonesia is distributed in Sumatra, Kalimantan, Java and surrounding islands. The high poaching of Indonesia pangolin to illegal trades are for traditional medicine ingredient in Indo-China region. The result is a decrease of qualitas and quantitas on wild population. This causes the current status of the pangolin is Critically Endangered and listed in Appendix II of CITES. The purpose of this study is to get the level of quality and genetic diversity and to know the origin of the pangolin confiscated from Java, Kalimantan and Sumatra based on the molecular analysis.  The wild samples are used as compare to them. Analysis through molecular technique using marker of control region (D-loop) mitochondrial DNA (mtDNA). The results based on haplotype and genetic distance shows a distinct difference in 19 haplotypes, with nine haplotypes from Kalimantan, seven from Java and three from Sumatera population. The avarage of genetic distance (d) between Kalimantan-Java is d=0.0121±0.0031 Kalimantan-Sumatera d=0.0123±0.0038 and Sumatera-Java d=0.0075±0038. The genetic distance of entire population is   d = 0.0148 ± 0.0035, with nucleotide diversity (π) 0.0146. The phylogenetic tree shows that the Kalimantan population is separated from   Sumatra and Java population then form a separate  clade.

Keywords: Manis javanica, diversity, genetic, Control region, distance

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