Source: SEAMEO BIOTROP's Research Grant | 2017
Primary selection is an effective method to develop SSR markers showing close kinship of several species in a taxonomic system. This study was aimed to measure diversity genetic of sengon resistant dan suceptible to boktor pest and gall rust disease based on microsatelit marker. Leaves samples of resistant and susceptible sengon to gall rust disease were collected from Kediri, and Sukabumi, while Leaves samples of resistant and susceptible sengon to boktor pest from Sumedang. Amplifications of eight SSR markers (Pafa02, Pafa03, Pafa04, Pafa05, Pafa06, Pafa07, Pafa08, and Pafa10) were tested on the sengon resistant and susceptible to gall rust from Sukabumi and Kediri and four SSR markers (Pafa06, Pafa07, Pafa08 dan Pafa10) were tested on the sengon resistant and susceptible to boktor from Sumedang. Results of analysis showed informative markers, i.e. average value of He = 0.479 to gall rust ; He 0.637 to boktor, respectively. The SSR markers could amplify most of sengon accessions used in this study. Molecular analysis showed high He value indicating the high genetic diversity of populations studied. The markers used could distinguish sengon accessions which were resistant to gall rust disease from the susceptible.