Secondary Metabolite From Mangrove Derived Endophytic Bacteria for Antioxidant and Toxicological Evaluation Using Brine Shrimp Model
S I Rahmawati1, F N Izzati, Y Hapsari, Yadi, E Septiana, F Rachman, Bustanussalam, S Nurhalimah, P Simanjuntak

Source: SEAMEO BIOTROP's Research Grant | 2019


The utilization of mangrove plants as traditional medicinal ingredients has long been utilized by the community for treatment of various diseases in favour of bioactive components from its secondary metabolites. Secondary metabolites from mangroves could act as an antioxidant to prevent oxidative stress. Mangrove may contain endophytic bacteria in its tissues that are capable of producing secondary metabolites. In theory, endophytic bacteria isolated from a plant can produce secondary metabolites similar to those of the original plants or even in relatively high numbers. In this research, seven mangroves species from Segara Anakan Lagoon, Cilacap, Central Java province, have been taken to investigate the antioxidant and toxicity activities of extracellular extracts of bacteria endophytic mangroves. Furthermore, isolation of endophytic microbes from seven mangroves had been done. This research succeeded to isolate 7 bacteria as mangroves endophytic bacteria. Meanwhile, the highest extraction yield was obtained by the bacteria isolated from fruits of Aegiceras corniculatum that is approximately 522,8 mg/100 mL media. The highest activities of antioxidant was obtained by bacteria isolated from leaves of Avicennia alba Blume. Furthermore, the highest activities of BSLA was obtained by extract from bacteria that isolated from fruits of Aegiceras corniculatum. From those result, we concluded that the important thing was antioxidant so extract from mangrove endophytic bacteria of Avicennia alba Blume leaves was purified. Fraction 28 chosen as an active and pure compound, then subjected to NMR analysis. As for the isolated bacteria from leaves of Avicennia alba Blume was identified using PCR.


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