Source: SEAMEO BIOTROP's Research Grant | 2021
Abstract:
The research aimed to study hydroponic mycorrhizal spore propagation techniques and compare them with
conventional techniques, to obtain quality AMF
inoculum formulations, to obtain appropriate storage techniques for AMF inoculum,
to study plant growth response to AMF inoculum and measure the
infectivity and effectiveness of
mycorrhizal-based biofertilizers. The research was conducted from March to November 2021 at the Biosystem
Landscape and Management (BLM) laboratory
and the SEAMEO BIOTROP Greenhouse. The study was designed using a factorial Completely Randomized Design
(CRD) with 3 factors. The first factor was
the type of formulation (F1 and F2), the best storage temperature (40oC
and 60oC), the type of mycorrhizal host plants used (Sorghum bicolor, Pueraria
javanica and Zea mays). The total experimental units
are 180 experimental units. The data were
statistically analyzed with SAS software version 9.0 and further tested using Duncan's
Multiple Range Test (DMRT) at an alpha level (α) 5%. The results showed
that both conventional and hydroponic techniques could be used to
produce AMF spores. The amount of
spore production depends on the ability of AMF infection. Glomus etunicatum has better root infection ability than Glomus fasciculatum. Hydroponic techniques produce better vegetative plant growth
than conventional techniques. The P3E_NFT treatment
resulted in the highest increase
in height (221.8
cm), the number of leaves (13), stem diameter (3 cm) and canopy dry
biomass (9.54 g). The use of PP
plastic (Polypropylen) can maintain the viability of spore density of 150/10g BIORISA after 1 month after
storage compared to HDPE plastic (High- Density
Polyethylene) which is only able to maintain spore density of 101/10g BIORISA. The results of analysis of
variance showed that giving the prototype mycorrhizal
fertilizer BIORISA code TZ60F1, TZ60F2, TZ40F1, TZ40F2 gave a significant effect on all plant growth
parameters (height, number of leaves and stem
diameter) after 4 weeks after presenting the soil medium.
The application of BIORISA on sand media is not optimal, so further research
is needed to increase the effectiveness of BIORISA,
especially when applied to degraded land.
Keywords: conventional, hydroponic, NFT, pot culture, Zea mays