Source: SEAMEO BIOTROP's Research Grant | 2021
The research aimed to study hydroponic mycorrhizal spore propagation techniques and compare them with conventional techniques, to obtain quality AMF inoculum formulations, to obtain appropriate storage techniques for AMF inoculum, to study plant growth response to AMF inoculum and measure the infectivity and effectiveness of mycorrhizal-based biofertilizers. The research was conducted from March to November 2021 at the Biosystem Landscape and Management (BLM) laboratory and the SEAMEO BIOTROP Greenhouse. The study was designed using a factorial Completely Randomized Design (CRD) with 3 factors. The first factor was the type of formulation (F1 and F2), the best storage temperature (40oC and 60oC), the type of mycorrhizal host plants used (Sorghum bicolor, Pueraria javanica and Zea mays). The total experimental units are 180 experimental units. The data were statistically analyzed with SAS software version 9.0 and further tested using Duncan's Multiple Range Test (DMRT) at an alpha level (α) 5%. The results showed that both conventional and hydroponic techniques could be used to produce AMF spores. The amount of spore production depends on the ability of AMF infection. Glomus etunicatum has better root infection ability than Glomus fasciculatum. Hydroponic techniques produce better vegetative plant growth than conventional techniques. The P3E_NFT treatment resulted in the highest increase in height (221.8 cm), the number of leaves (13), stem diameter (3 cm) and canopy dry biomass (9.54 g). The use of PP plastic (Polypropylen) can maintain the viability of spore density of 150/10g BIORISA after 1 month after storage compared to HDPE plastic (High- Density Polyethylene) which is only able to maintain spore density of 101/10g BIORISA. The results of analysis of variance showed that giving the prototype mycorrhizal fertilizer BIORISA code TZ60F1, TZ60F2, TZ40F1, TZ40F2 gave a significant effect on all plant growth parameters (height, number of leaves and stem diameter) after 4 weeks after presenting the soil medium. The application of BIORISA on sand media is not optimal, so further research is needed to increase the effectiveness of BIORISA, especially when applied to degraded land.
Keywords: conventional, hydroponic, NFT, pot culture, Zea mays