Source: SEAMEO BIOTROP's Research Grant | 2021
Abstract:
Objective
The aim of this study is to explore the diversity of toxicigenic
and non-toxigenic molecular
profiles of A. flavus from Indonesia through:
1. Characterization of the molecular profile of toxigenic
and non-toxigenic A. flavus
using twenty-nine genes involved in aflatoxin biosynthesis using PCR method.
2. Validation of the
potential for aflatoxin production using
HPLC.
3.
Exploration of specific candidate
gene between toxigenic
and non-toxigenic A. flavus
from Indonesia.
Expected Output
The expected output of this study will be a database of
molecular profiles of toxigenic and non-toxigenic
A. flavus based on aflatoxin
biosynthesis genes, which will be the basis for the development of molecular
Conclusion
Molecular characterization of A. flavus strains from Indonesia using 29 genes involved in aflatoxin biosynthesis has been able to
provide an overview of the molecular profiles of toxigenic and non-toxigenic A.
flavus. All strains were confirmed as A.
flavus through molecular
identification, the ability to produce aflatoxins has also been confirmed
through HPLC assays. Twenty-nine
pairs of primers were successfully amplified, and most of them produced single amplicon in toxigenic
strains. Meanwhile, some low toxigenic and non- toxigenic strains produced a multiband amplicon pattern.
Deletion variations in several target
genes were found in both toxigenic and non-toxigenic strains, so that the
twenty-nine target genes were not
able to distinguish the toxigenicity of A.
flavus tested. This study also succeeded
in characterizing 2 non-toxigenic candidates of A. flavus which lost most of the
aflatoxin biosynthetic genes, these characteristics are very useful for
use as biocontrol agents in the field against toxigenic
A. flavus.
markers for the
detection of toxigenic strains of A.
flavus in agricultural commodities.
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