Source: SEAMEO BIOTROP's Research Grant | 2021



The aim of this study is to explore the diversity of toxicigenic and non-toxigenic molecular profiles of A. flavus from Indonesia through:

1.      Characterization of the molecular profile of toxigenic and non-toxigenic A. flavus

using twenty-nine genes involved in aflatoxin biosynthesis using PCR method.

2.      Validation of the potential for aflatoxin production using HPLC.

3.      Exploration of specific candidate gene between toxigenic and non-toxigenic A. flavus from Indonesia.




Expected Output

The expected output of this study will be a database of molecular profiles of toxigenic and non-toxigenic A. flavus based on aflatoxin biosynthesis genes, which will be the basis for the development of molecular



Molecular characterization of A. flavus strains from Indonesia using 29 genes involved in aflatoxin biosynthesis has been able to provide an overview of the molecular profiles of toxigenic and non-toxigenic A. flavus. All strains were confirmed as A. flavus through molecular identification, the ability to produce aflatoxins has also been confirmed through HPLC assays. Twenty-nine pairs of primers were successfully amplified, and most of them produced single amplicon in toxigenic strains. Meanwhile, some low toxigenic and non- toxigenic strains produced a multiband amplicon pattern. Deletion variations in several target genes were found in both toxigenic and non-toxigenic strains, so that the twenty-nine target genes were not able to distinguish the toxigenicity of A. flavus tested. This study also succeeded in characterizing 2 non-toxigenic candidates of A. flavus which lost most of the aflatoxin biosynthetic genes, these characteristics are very useful for use as biocontrol agents in the field against toxigenic A. flavus.

 markers for the detection of toxigenic strains of A. flavus in agricultural commodities.


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