Source: SEAMEO BIOTROP's Research Grant | 2021
This study explores the potential marker fertility in the sperm of bulls of various local cattle breeds. This sperm molecular analysis to (1) describes the RNA fertility marker gene expression, (2) accurately can determine the potential and the single nucleotide polymorphism from the HSP 70-2 (HSP A2), and OPN as fertility marker, (3) Describe the profiles and interactions of sperm proteins that modulate fertilization.
The results of this study are expected to be able to provide several molecular marker information related to bulls fertility, which is essential as a basis for selection and reselection for determining superior bulls that are accurate, have high fertility, and as a policy reference in the bulls refusal process used by the Indonesian Center for Artificial Insemination. Besides, it is also a measure to preserve Madura cattle as local Indonesian germplasm.
The Madura bulls could be grouped into three fertility rates based on the field conception rate data (iSIKHNAS data): highly fertile, medium fertile, and low fertile. The sperm protein with an MWs 75.35 kDa and 5.36 kDa is predicted as HSP70 and PRM1 sperm protein biomarker that abundance in the high fertile of Madura bulls. They were expected as a specific protein biomarker that influences the Madura bull’s fertility. Thus, the presence of specific sperm proteins and the viability of normal sperm are determiners of sperm fertilization abilities.
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